pbmc generation  (Cellular Technology Ltd)

Journal: Cell Genomics

Article Title: Single-cell transcriptome landscape of circulating CD4 + T cell populations in autoimmune diseases

doi: 10.1016/j.xgen.2023.100473

Figure Lengend Snippet:

Article Snippet: We collected scRNA-seq data from PBMC generated by 10x platforms, Seq-Well or SPLiT-seq (Parse Biosciences WT Mega) , , , , , , , , , , , , , , , , , , , , , , , ( ).

Techniques: Binding Assay, Functional Assay, Recombinant, Staining, Amplification, Software

Journal: NanoImpact

Article Title: Oxidative Stress, DNA Methylation, and Telomere Length Changes in Peripheral Blood Mononuclear Cells after Pulmonary Exposure to Metal-Rich Welding Nanoparticles

doi: 10.1016/j.impact.2017.01.001

Figure Lengend Snippet: PBMCs isolated at 4 h, 14 h, 1 d, 3 d, 10 d, and 30 d after ITI exposure to 2.0 mg/rat of MMA-SS or GMAMS welding fumes. Vehicle controls received saline. (n = 4–6; values are means ± standard error).

Article Snippet: The PBMCs isolated from the GMA-MS group had slightly increased P-HNE adducts when compared to the controls. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Figure 5. caption a7 ROS generation (DHE; red fluorescence) by PBMCs isolated from Sprague-Dawley rats at 24 h after 2.0 mg/rat ITI exposure to MMA-SS or GMA-MS welding fumes.

Techniques: Isolation, Saline

Journal: NanoImpact

Article Title: Oxidative Stress, DNA Methylation, and Telomere Length Changes in Peripheral Blood Mononuclear Cells after Pulmonary Exposure to Metal-Rich Welding Nanoparticles

doi: 10.1016/j.impact.2017.01.001

Figure Lengend Snippet: ROS generation (DHE; red fluorescence) by PBMCs isolated from Sprague-Dawley rats at 24 h after 2.0 mg/rat ITI exposure to MMA-SS or GMA-MS welding fumes. Vehicle controls received saline.

Article Snippet: The PBMCs isolated from the GMA-MS group had slightly increased P-HNE adducts when compared to the controls. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Figure 5. caption a7 ROS generation (DHE; red fluorescence) by PBMCs isolated from Sprague-Dawley rats at 24 h after 2.0 mg/rat ITI exposure to MMA-SS or GMA-MS welding fumes.

Techniques: Fluorescence, Isolation, Saline

Journal: NanoImpact

Article Title: Oxidative Stress, DNA Methylation, and Telomere Length Changes in Peripheral Blood Mononuclear Cells after Pulmonary Exposure to Metal-Rich Welding Nanoparticles

doi: 10.1016/j.impact.2017.01.001

Figure Lengend Snippet: P-HNE adduct formation (green fluorescence; FTIC) in PBMCs isolated from Sprague-Dawley rats at 24 h after ITI exposure to 2.0 mg/rat of MMA-SS or GMA-MS welding fumes. Vehicle controls received saline. DAPI blue fluorescence indicates nucleus staining.

Article Snippet: The PBMCs isolated from the GMA-MS group had slightly increased P-HNE adducts when compared to the controls. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Figure 5. caption a7 ROS generation (DHE; red fluorescence) by PBMCs isolated from Sprague-Dawley rats at 24 h after 2.0 mg/rat ITI exposure to MMA-SS or GMA-MS welding fumes.

Techniques: Fluorescence, Isolation, Saline, Staining

Journal: NanoImpact

Article Title: Oxidative Stress, DNA Methylation, and Telomere Length Changes in Peripheral Blood Mononuclear Cells after Pulmonary Exposure to Metal-Rich Welding Nanoparticles

doi: 10.1016/j.impact.2017.01.001

Figure Lengend Snippet: DNA methylation was measured by the production of 5-methylcytosine (5-mC) in PBMCs isolated at (A) 1 d, (B) 3 d, (C) 10 d, and (D) 30 d after ITI exposure to 2.0 mg/rat of MMA-SS or GMA-MS welding fumes. Vehicle controls received saline. (n = 4–6; values are means ± standard error).

Article Snippet: The PBMCs isolated from the GMA-MS group had slightly increased P-HNE adducts when compared to the controls. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Figure 5. caption a7 ROS generation (DHE; red fluorescence) by PBMCs isolated from Sprague-Dawley rats at 24 h after 2.0 mg/rat ITI exposure to MMA-SS or GMA-MS welding fumes.

Techniques: DNA Methylation Assay, Isolation, Saline

Journal: NanoImpact

Article Title: Oxidative Stress, DNA Methylation, and Telomere Length Changes in Peripheral Blood Mononuclear Cells after Pulmonary Exposure to Metal-Rich Welding Nanoparticles

doi: 10.1016/j.impact.2017.01.001

Figure Lengend Snippet: Telomere length of PBMCs isolated at (A) 1 d, (B) 3 d, (C) 10 d, and (D) 30 d after ITI exposure to 2.0 mg/rat of MMA-SS or GMA-MS welding fumes. Vehicle controls received saline. (n = 4–6; values are means ± standard error; *significantly different from saline control and GMA-MS groups, p<0.05). The relative telomere length was measured by comparing the ratio of telomere repeat copy number (T) and single gene copy number (S), expressed as telomere length (T/S) ratio.

Article Snippet: The PBMCs isolated from the GMA-MS group had slightly increased P-HNE adducts when compared to the controls. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Figure 5. caption a7 ROS generation (DHE; red fluorescence) by PBMCs isolated from Sprague-Dawley rats at 24 h after 2.0 mg/rat ITI exposure to MMA-SS or GMA-MS welding fumes.

Techniques: Isolation, Saline, Control